P-49: Effect of Crab Shell HydroalcoholicExtract Induced Apoptosis in ProstateCancer Cell Line

Background: Prostate cancer is the second most common cancer in men worldwide and the third most common cancer in Iranian men. Chemotherapy is used to treat this cancer, but it has toxic effect. Natural compounds found in marine organisms and plants have anticancer considered. The aim of the present study was to investigate the effect of crab shell extract on induction of apoptosis in prostate cancer cells (LNcap). Materials and Methods: Crab shell was prepared and powdered. Then the hydroalcoholic method was extracted. LNcap was used in this study. The extract was prepared 5 concentrations (100, 200, 400, 800 and 1000 μg /ml) and within 72 hours were effective on cells. In the above study to evaluate apoptosis, cells were stained with TUNEL method, and were photographed with a fluorescence microscope. Data were analyzed by ANOVA and p<0.05 was considered significant. Results: Apoptosis was evaluated by TUNEL staining method on LNcap. Index of apoptosis in control group and 100, 200, 400, 800 and 1000 μg/ml extracts were 1.73, 3.16, 4.17, 6.86, 17.77 and 19.11, respectively. This results in 400 μg /ml group of extract in comparison with control group was significant (p<0.05) and in groups of 800 and 1000 μg /ml of crab shell extract in comparison with the control group was significant (p<0.001). Conclusion: Crab shell extract induced apoptosis in LNcap dose dependently.